Effect of Gamma Radiation and Gibberellic Acid on Germination and Alkaloid Production in Atropa belladonna l

This investigation was carried out to improve the germination percentage and alkaloid 1 production in M seeds (irradiated seeds with 50, 80, 110 and 150 Gy) of Atropa belladonna L. by using and different concentrations of gibberellic acid (20,40,60,80,100 and 120 ppm). There was an increase in seed germination percentage by increasing gamma-ray doses until 150 Gy which gave 3 decrease in seed germination percentage, and with regard to the effect of GA on seed germination 3 percentage, there was increasing in this percentage by increasing GA concentration until 100 ppm. 2 The best interaction was between 110 Gy and 100 ppm which was the highest percentage. At, M , a new mutation in seed colour (Red seeds) for almost mutants under study would be considered as a solution for genetic germination problems, that the germination percentage reached to 100 % . Three 2 promising high alkaloids mutants were selected from M , M-11-1, M-11-2 and M-15-1.